Separation: The cell stage interacts While using the stationary period from the column plus the analytes during the sample. This interaction influences how promptly Every analyte travels throughout the column, resulting in their separation.
Cellular period range: The mobile period performs a vital position in separating analytes. Opt for a cellular stage that interacts differently Together with the analytes, permitting for improved separation. Experiment with distinctive solvent combinations or modify the pH from the cell period.
예를 들어 설탕과 같이 물에 녹기 쉬운 물질을 첨가했을 때 설탕은 기름층에 거의 녹지 않으므로 물층에 많이 존재하게 됩니다. 반대로 식용유와 같이 헥산에 녹기 쉬운 용질을 첨가했을 때는 물층보다 기름층에 많이 존재합니다. 이와같이, 설탕과 식용유는 물과 헥산의 두 상 사이의 존재의 비율(=분배 비율)이 크게 다르기 때문에, 만약 당신과 이 분액깔대기에서 설탕만을 분리하고 싶다면, 분액깔대기에서 물층만을 꺼내 물을 증류시키면 설탕만을 얻을 수 있습니다.
Recall, consulting your instrument manual along with the company's technical assist will also be worthwhile methods when troubleshooting specific difficulties together with your HPLC system.
The data acquisition system data and analyses the detector signals, making it possible for chemical substances to get quantified based on their own peak areas within the chromatogram.
The pump is accountable for offering the mobile period at a constant flow charge. This makes sure that the cellular phase is continuously fed into the column.
The detector here displays the eluent and generates a sign, which is often in the form of the chromatogram, which happens to be a graphical illustration of compound focus over time.
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스포츠 도핑에서 약물 검사까지 법의독성학 응용 분야에 적용되는 방법에 대해 알아보세요.
Usual-section: Separates according to polarity. Analytes with higher polarity interact far more Using the polar stationary stage and elute later on.
. Solvent triangle for optimizing a reversed-phase HPLC separation. The 3 blue circles demonstrate cellular phases consisting check here of an organic and natural solvent and water.
The choice to start with acetonitrile is arbitrary—we can easily equally as easily decide on to begin with methanol or with tetrahydrofuran.
The choice of detector depends upon the precise desires of your Assessment, looking at elements like sensitivity, selectivity, and compatibility Together with the cellular section.
An inside typical is essential when using HPLC–MS as the interface concerning the HPLC and the mass spectrometer does not allow for your reproducible transfer in the column’s eluent in to the MS’s ionization chamber.